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Dengue virus NS5 protein antibody [GT361]

Anti-Dengue virus NS5 protein antibody [GT361] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629447
Anti-Dengue virus NS5 protein antibody [GT361] used in Western Blot (WB). GTX629447
Anti-Dengue virus NS5 protein antibody [GT361] used in Western Blot (WB). GTX629447
Anti-Dengue virus NS5 protein antibody [GT361] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629447
Anti-Dengue virus NS5 protein antibody [GT361] used in Western Blot (WB). GTX629447
Anti-Dengue virus NS5 protein antibody [GT361] used in Western Blot (WB). GTX629447

Cat. No. GTX629447

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT361

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Dengue virus 2, Dengue virus 3, Dengue virus 4
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus NS5 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

Dengue virus 2 infected BHK21 cells , Dengue virus type 3 infected BHK-21 cells

Product Note

This antibody does not cross-react with JEV Envelope protein.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus Type 2 NS5 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888113

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Non-structural protein 5 , Dengue Virus NS5 , DENV Non-structural protein 5 , DENV NS5

Research Area

DATA IMAGES

Anti-Dengue virus NS5 protein antibody [GT361] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629447

GTX629447 ICC/IF Image

Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.
Green: NS5 (Dengue virus 2) protein stained by Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS5 protein antibody [GT361] used in Western Blot (WB). GTX629447

GTX629447 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus NS5 protein antibody [GT361] used in Western Blot (WB). GTX629447

GTX629447 WB Image

Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
10% SDS-PAGE
Dengue virus NS5 protein antibody [GT361] (GTX629447) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus NS5 protein antibody [GT361] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629447

GTX629447 ICC/IF Image

Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde.
Green: NS5 (Dengue virus 2) protein stained by Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS5 protein antibody [GT361] used in Western Blot (WB). GTX629447

GTX629447 WB Image

Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
7.5% SDS-PAGE
Dengue virus NS5 protein antibody [GT361] (GTX629447) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS5 protein antibody [GT361] used in Western Blot (WB). GTX629447

GTX629447 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Butler, M et al. Viruses 2020; 12 (6) : E654 Cyclin-Dependent Kinases 8 and 19 Regulate Host Cell Metabolism During Dengue Virus Serotype 2 Infection.
Application : WB
Xie X et al. Cell Host Microbe 2019; 26 (5) : 606-622.e8 Dengue NS2A Protein Orchestrates Virus Assembly.
Application : WB
Ngo AM et al. Elife 2019; 8 The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.
Application : WB
Lim SP et al. PLoS Pathog 2016; 12 (8) : e1005737 Potent Allosteric Dengue Virus NS5 Polymerase Inhibitors: Mechanism of Action and Resistance Profiling.
Application : WB
Alves RP et al. Clin Vaccine Immunol 2016; (Epub) Production of a recombinant Dengue virus 2 NS5 protein and the potential use as a vaccine antigen.
Application : WB
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169