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Dengue virus prM protein antibody

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093

Cat. No. GTX128093

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Dengue virus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Dengue virus type 2 infected BHK-21

Product Note

This antibody was raised against Dengue virus 2 prM protein, which may cross react with prM protein of Dengue virus 1/3/4 but does not recognize with prM protein of JEV.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus PrM protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885700

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Premembrane , Dengue Virus PrM , DENV Premembrane , DENV PrM

Research Area

DATA IMAGES

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093

GTX128093 WB Image

prM (Dengue virus 2) antibody detects prM (Dengue virus 2) protein by western blot analysis.
A.30 μg BHK-21 whole cell lysate/extract
B.30 μg whole cell lysate/extract of Dengue virus 2 infected BHK-21 cells
C.30 μg whole cell lysate/extract of JEV virus infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus 2) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093

GTX128093 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus prM protein antibody (GTX128093) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects PrM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by PrM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093

GTX128093 WB Image

The data was published in the journal PLoS Pathog in 2013. PMID: 24348242

REFERENCE

REVIEW

Dengue virus prM protein antibody Cat. No. GTX128093

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 25-Dec-2019
Score :
Application Tested : WB
Sample Species : DENV2
Sample : Huh7 cells
Amount used : 10 μg
Blocking : 2.5% non-fat skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Huh7 whole cell lysate; Lane 2: Dengue virus type II NGC strain infected.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169