GeneTex
  • Country / Location Selection

United States (US)

Deptor antibody [N1C3]

Anti-Deptor antibody [N1C3] used in Western Blot (WB). GTX116540
Anti-Deptor antibody [N1C3] used in Western Blot (WB). GTX116540

Cat No. GTX116540

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , MCF-7

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Deptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163178

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DEP domain containing MTOR interacting protein , DEP.6 , DEPDC6

Background

Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.

Database

Research Area

DATA IMAGES

Anti-Deptor antibody [N1C3] used in Western Blot (WB). GTX116540

GTX116540 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Deptor antibody [N1C3] (GTX116540) diluted at 1:1000.

Anti-Deptor antibody [N1C3] used in Western Blot (WB). GTX116540

GTX116540 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX116540 diluted at 1:1000

REFERENCE

There are currently no references for Deptor antibody [N1C3] (GTX116540). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Deptor antibody [N1C3] (GTX116540). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf