PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:200 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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Not tested in other applications.
Calculated MW
Positive Control
Appendix: Smooth muscle cells in the lamina muscularis mucosae and muscularis propria must show a strong cytoplasmic staining while staining should be at least moderate in most smooth muscle cells of blood vessels and in dispersed myofibroblasts.
Negative Control
Appendix: Desmin staining should not be seen in lymphocytes and epithelial cells.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human desmin protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
desmin , CDCD3 , CMD1F , CSM1 , CSM2 , LGMD1D , LGMD1E , LGMD2R
Cellular Localization
Cytoplasm, myofibril, sarcomere, Z line , Cytoplasm , Nucleus
Background
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04410 IHC-P Image
IHC-P analysis of human stomach Gastric adenocarcinoma tissue section using GTX04410 Desmin antibody [MSVA-651M] HistoMAX™. Stomach Gastric adenocarcinoma invading desmin positive smooth muscle fibres.
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GTX04410 IHC-P Image
IHC-P analysis of human rectum tissue section using GTX04410 Desmin antibody [MSVA-651M] HistoMAX™. Strong desmin staining of lamina muscularis mucosae.
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GTX04410 IHC-P Image
IHC-P analysis of human rhabdomyosarcoma tissue section using GTX04410 Desmin antibody [MSVA-651M] HistoMAX™. Soft tissue Strong desmin positivity of all tumor cells of a rhabdomyosarcoma.
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REFERENCE
There are currently no references for Desmin antibody [MSVA-651M] HistoMAX™ (GTX04410). Be the first to share your publications with this product.
REVIEW
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