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Desmin antibody

Orthogonal
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557

Cat. No. GTX103557

Reference 17

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat, Cat, Dog, Chicken, Pig
Package
100 μl ($399),
25 μl ($169)
See all Desmin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

A549 , HCT116 , 50 μg mouse muscle , Rat2

Predict Reactivity

Rabbit, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Desmin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950112

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

desmin , CDCD3 , CMD1F , CSM1 , CSM2 , LGMD1D , LGMD1E , LGMD2R

Cellular Localization

Cytoplasm

Background

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects Desmin protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:1000

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects DES protein by Western blot analysis.
A. 50 μg mouse muscle lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:10000

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects DES protein by Western blot analysis.
A. 30 μg A549 whole cell lysate/extract | B. 30 μg HCT116 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:3000

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103557

GTX103557 ICC/IF Image

Desmin antibody detects Desmin protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog malignant soft tissue sarcoma.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytosol on mouse skeletal muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skeletal muscle.
Desmin antibody (GTX103557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects DES protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Desmin antibody (GTX103557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Orthogonal

The data was published in the journal PLoS One in 2016.PMID: 27589391

REFERENCE

Application Reference

LA Skelton et al. Exp Eye Res 2023; 234 : 109585 Retinal gliosis and phenotypic diversity of intermediate filament induction and remodeling upon acoustic blast overpressure (ABO) exposure to the rat eye
Application : WB, IHC-Fr
Reactivity : Rat
Yeojin Lee et al. Sci Adv 2022; 8 (11) : eabm7688 Hematopoietic stem cells temporally transition to thrombopoietin dependence in the fetal liver.
Application : IHC-Fr
Reactivity : Mouse
Lee Y et al. J Exp Med 2021; 218 (3) Hepatic stellate and endothelial cells maintain hematopoietic stem cells in the developing liver.
Weber B et al. Sci Rep 2021; 11 (1) : 8503 Early myocardial damage (EMD) and valvular dysfunction after femur fracture in pigs.
Application : IHC-P
Reactivity : Pig
Lackner I et al. J Orthop Res 2020; 38 (12) : 2608-2618 Reaming of femoral fractures with different reaming irrigator aspirator systems shows distinct effects on cardiac function after experimental polytrauma.
Application : IHC-P
Reactivity : Pig
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Application Reference
LA Skelton et al. Exp Eye Res 2023; 234:109585 Retinal gliosis and phenotypic diversity of intermediate filament induction and remodeling upon acoustic blast overpressure (ABO) exposure to the rat eye
Application : WB, IHC-Fr
Reactivity : Rat
Yeojin Lee et al. Sci Adv 2022; 8 (11):eabm7688 Hematopoietic stem cells temporally transition to thrombopoietin dependence in the fetal liver.
Application : IHC-Fr
Reactivity : Mouse
Lee Y et al. J Exp Med 2021; 218 (3) Hepatic stellate and endothelial cells maintain hematopoietic stem cells in the developing liver.
Weber B et al. Sci Rep 2021; 11 (1):8503 Early myocardial damage (EMD) and valvular dysfunction after femur fracture in pigs.
Application : IHC-P
Reactivity : Pig
Lackner I et al. J Orthop Res 2020; 38 (12):2608-2618 Reaming of femoral fractures with different reaming irrigator aspirator systems shows distinct effects on cardiac function after experimental polytrauma.
Application : IHC-P
Reactivity : Pig
Chen YH et al. Sci Rep 2019; 9 (1):9919 Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells.
Application : ICC/IF
Reactivity : Human
Foertsch S et al. Brain Behav Immun 2019; (Epub) Sensory contact to the stressor prevents recovery from structural and functional heart damage following psychosocial trauma.
Application : IHC-P
Reactivity : Mouse
Li YK et al. J Dig Dis 2019; (Epub) Increased Cthrc1 expression in primary biliary cholangitis.
Application : IHC-P
Reactivity : Human
Taylor L Barnes et al. Translational Animal Science 2018; 2 (suppl_1):S27-S30 Impaired muscle stem cell function in cows with high concentrations of * in their follicular fluid.
Application : FACS
Reactivity : Human
Fang CC et al. Sci Rep 2018; 8 (1):13802 AAV serotype 8-mediated liver specific GNMT expression delays progression of hepatocellular carcinoma and prevents carbon tetrachloride-induced liver damage.
Application : IHC-P
Reactivity : Mouse
Cargnoni A et al. J Cell Mol Med 2017; (Epub) Effect of human amniotic epithelial cells on pro-fibrogenic resident hepatic cells in a rat model of liver fibrosis.
Application : IHC-P
Reactivity : Human
Lao S et al. Mol Med Rep 2017; 16 (1):507-514 A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells.
Application : WB
Reactivity : Human
Klein S et al. PLoS One 2016; 11 (9):e0162144 Novel Rat Model of Repetitive Portal Venous Embolization Mimicking Human Non-Cirrhotic Idiopathic Portal Hypertension.
Application : WB
Reactivity : Rat
Hara Y et al. Anat Rec (Hoboken) 2016; (Epub) Development of the heart endocardium at an early stage of chick embryos evaluated at light- and electron-microscopic levels.
Application : IHC-P
Reactivity : Chicken
Treskes P et al. J Thorac Cardiovasc Surg 2015; 149 (1):348-56 Preconditioning of skeletal myoblast-based engineered tissue constructs enables functional coupling to myocardium in-vivo.
Application : IHC-Fr
Reactivity : Mouse
Ai F et al. Biotechnol Lett 2015; (Epub) Restoration of miR-98 relieves the inhibitory effect of nicotine on the differentiation of P19 cells into cardiomyocytes.
Application : WB
Reactivity : Mouse
Kiss E et al. Am J Pathol 2013; 182 (3):727-41 Lipid droplet accumulation is associated with an increase in hyperglycemia-induced renal damage: prevention by liver X receptors.
Application : IHC-P
Reactivity : Mouse
SDS
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Thimerosal.pdf
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