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Desmin antibody

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557

Cat No. GTX103557

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS
Reactivity Human, Mouse, Rat, Chicken
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

RD , mouse muscle

Predict Reactivity

Rabbit, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Desmin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

desmin,CDCD3,CMD1F,CSM1,CSM2,LGMD1D,LGMD1E,LGMD2R

Cellular Localization

Cytoplasm

Background

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects DES protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Desmin antibody (GTX103557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects DES protein by Western blot analysis.
A. 30 µg A549 whole cell lysate/extract | B. 30 µg HCT116 whole cell lysate/extract
B. 30 µg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:3000

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects DES protein by Western blot analysis.
A. 30 µg RD whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:500

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytosol on mouse skeletal muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skeletal muscle.
Desmin antibody (GTX103557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects DES protein by Western blot analysis.
A. 50 µg mouse muscle lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:10000

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytosol on mouse smooth muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Desmin antibody (GTX103557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects Desmin protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:1000

REFERENCE
REVIEW
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Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169