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Desmin antibody

Orthogonal
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

Cat. No. GTX103557

Reference 16
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat, Cat, Dog, Chicken, Pig
Package
100 μl ($399),
25 μl ($169)
See all Desmin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

A549 , HCT116 , 50 μg mouse muscle , Rat2

Predict Reactivity

Rabbit, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7)

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Desmin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950112

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

desmin , CDCD3 , CMD1F , CSM1 , CSM2 , LGMD1D , LGMD1E , LGMD2R

Cellular Localization

Cytoplasm

Background

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103557

GTX103557 ICC/IF Image

Desmin antibody detects Desmin protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog malignant soft tissue sarcoma.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytosol on mouse skeletal muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skeletal muscle.
Desmin antibody (GTX103557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects DES protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Desmin antibody (GTX103557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects Desmin protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:1000

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects DES protein by Western blot analysis.
A. 50 μg mouse muscle lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:10000

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects DES protein by Western blot analysis.
A. 30 μg A549 whole cell lysate/extract | B. 30 μg HCT116 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:3000

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yeojin Lee et al. Sci Adv 2022; 8 (11) : eabm7688 Hematopoietic stem cells temporally transition to thrombopoietin dependence in the fetal liver.
Application : IHC-Fr
Reactivity : Mouse
Lee Y et al. J Exp Med 2021; 218 (3) Hepatic stellate and endothelial cells maintain hematopoietic stem cells in the developing liver.
Weber B et al. Sci Rep 2021; 11 (1) : 8503 Early myocardial damage (EMD) and valvular dysfunction after femur fracture in pigs.
Application : IHC-P
Reactivity : Pig
Lackner I et al. J Orthop Res 2020; 38 (12) : 2608-2618 Reaming of femoral fractures with different reaming irrigator aspirator systems shows distinct effects on cardiac function after experimental polytrauma.
Application : IHC-P
Reactivity : Pig
Li YK et al. J Dig Dis 2019; (Epub) Increased Cthrc1 expression in primary biliary cholangitis.
Application : IHC-P
Reactivity : Human
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Submit a Reference

REVIEW

Desmin antibody Cat. No. GTX103557

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27589391
Submit a Review
Application Reference
Yeojin Lee et al. Sci Adv 2022; 8 (11):eabm7688 Hematopoietic stem cells temporally transition to thrombopoietin dependence in the fetal liver.
Application : IHC-Fr
Reactivity : Mouse
Lee Y et al. J Exp Med 2021; 218 (3) Hepatic stellate and endothelial cells maintain hematopoietic stem cells in the developing liver.
Weber B et al. Sci Rep 2021; 11 (1):8503 Early myocardial damage (EMD) and valvular dysfunction after femur fracture in pigs.
Application : IHC-P
Reactivity : Pig
Lackner I et al. J Orthop Res 2020; 38 (12):2608-2618 Reaming of femoral fractures with different reaming irrigator aspirator systems shows distinct effects on cardiac function after experimental polytrauma.
Application : IHC-P
Reactivity : Pig
Chen YH et al. Sci Rep 2019; 9 (1):9919 Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells.
Application : ICC/IF
Reactivity : Human
Foertsch S et al. Brain Behav Immun 2019; (Epub) Sensory contact to the stressor prevents recovery from structural and functional heart damage following psychosocial trauma.
Application : IHC-P
Reactivity : Mouse
Li YK et al. J Dig Dis 2019; (Epub) Increased Cthrc1 expression in primary biliary cholangitis.
Application : IHC-P
Reactivity : Human
Taylor L Barnes et al. Translational Animal Science 2018; 2 (suppl_1):S27-S30 Impaired muscle stem cell function in cows with high concentrations of * in their follicular fluid.
Application : FACS
Reactivity : Human
Fang CC et al. Sci Rep 2018; 8 (1):13802 AAV serotype 8-mediated liver specific GNMT expression delays progression of hepatocellular carcinoma and prevents carbon tetrachloride-induced liver damage.
Application : IHC-P
Reactivity : Mouse
Cargnoni A et al. J Cell Mol Med 2017; (Epub) Effect of human amniotic epithelial cells on pro-fibrogenic resident hepatic cells in a rat model of liver fibrosis.
Application : IHC-P
Reactivity : Human
Lao S et al. Mol Med Rep 2017; 16 (1):507-514 A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells.
Application : WB
Reactivity : Human
Klein S et al. PLoS One 2016; 11 (9):e0162144 Novel Rat Model of Repetitive Portal Venous Embolization Mimicking Human Non-Cirrhotic Idiopathic Portal Hypertension.
Application : WB
Reactivity : Rat
Hara Y et al. Anat Rec (Hoboken) 2016; (Epub) Development of the heart endocardium at an early stage of chick embryos evaluated at light- and electron-microscopic levels.
Application : IHC-P
Reactivity : Chicken
Treskes P et al. J Thorac Cardiovasc Surg 2015; 149 (1):348-56 Preconditioning of skeletal myoblast-based engineered tissue constructs enables functional coupling to myocardium in-vivo.
Application : IHC-Fr
Reactivity : Mouse
Ai F et al. Biotechnol Lett 2015; (Epub) Restoration of miR-98 relieves the inhibitory effect of nicotine on the differentiation of P19 cells into cardiomyocytes.
Application : WB
Reactivity : Mouse
Kiss E et al. Am J Pathol 2013; 182 (3):727-41 Lipid droplet accumulation is associated with an increase in hyperglycemia-induced renal damage: prevention by liver X receptors.
Application : IHC-P
Reactivity : Mouse
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169