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Desmin antibody

Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in Western Blot (WB). GTX103557
Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

Cat No. GTX103557

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

Human, Mouse, Rat, Chicken, Pig
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

RD , mouse muscle

Predict Reactivity

Rabbit, Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Desmin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950112

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

desmin , CDCD3 , CMD1F , CSM1 , CSM2 , LGMD1D , LGMD1E , LGMD2R

Cellular Localization

Cytoplasm

Background

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects DES protein by Western blot analysis.
A. 30 μg A549 whole cell lysate/extract | B. 30 μg HCT116 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:3000

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects Desmin protein at cytosol on mouse skeletal muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skeletal muscle.
Desmin antibody (GTX103557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects DES protein by Western blot analysis.
A. 50 μg mouse muscle lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:10000

Anti-Desmin antibody used in Western Blot (WB). GTX103557

GTX103557 WB Image

Desmin antibody detects Desmin protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:1000

Anti-Desmin antibody used in IHC (Paraffin sections) (IHC-P). GTX103557

GTX103557 IHC-P Image

Desmin antibody detects DES protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Desmin antibody (GTX103557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

Desmin antibody Cat No. GTX103557

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27589391
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169