For IF: Use at an assay dependent dilution. For IHC-Fr: Use at a concentration of 20-50 μg/ml. For IP: Use at a concentration of 5-10 μg/ml. For WB: Use at a concentration of 1-3 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.
Differentiated keratinocytes and Desmoglein 1 transfected cells
This antibody does not cross-react with Desmoglein 2 or Desmoglein 3.
Phosphate-buffered saline, pH 7.2, containing 0.1% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
Intracellular domain of Desmoglein 1 (Human).
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. Desmoglein 1 has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus.