GeneTex
United States (US)

Desmoglein 1 antibody [3G131]

Cat No. GTX14414

Host Mouse
Clonality Monoclonal
Clone Name 3G131
Isotype IgG1
Application WB, ICC/IF, IHC-Fr, IP
Reactivity Human
APPLICATION

Application Note

For IF: Use at an assay dependent dilution. For IHC-Fr: Use at a concentration of 20-50 μg/ml. For IP: Use at a concentration of 5-10 μg/ml. For WB: Use at a concentration of 1-3 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

114 kDa. ( Note )

Positive Control

Differentiated keratinocytes and Desmoglein 1 transfected cells

Specificity/Sensitivity

This antibody does not cross-react with Desmoglein 2 or Desmoglein 3.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Intracellular domain of Desmoglein 1 (Human).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Desmoglein 1,Cdhf4,Dg1,Dsg,Epkhe,Epkhia,Ppks1,Sppk1,Dsg1

Cellular Localization

Cell Membrane

Background

Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. Desmoglein 1 has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus.

Database

Package List Price ($)
$ 319