APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1-2ug/ml |
| 0.5-1ug/ml for 30 minutes at RT |
|
Note :
IHC-P
Boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes
|
Not tested in other applications.
Calculated MW
Specificity/Sensitivity
Recognizes a protein of ~150kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.
PROPERTIES
Form
Liquid
Buffer
10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml
Antigen Species
Human
Immunogen
Recombinant human desmoglein-1 protein fragment corresponding to intracellular domain (exact sequence is proprietary)
Purification
Ab purified from Bioreactor Concentrate by Protein A/G
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Desmoglein 1 , Cdhf4 , Dg1 , Dsg , Epkhe , Epkhia , Ppks1 , Sppk1 , Dsg1
Background
This gene encodes a member of the desmoglein protein subfamily. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmoglein family members on chromosome 18. The encoded protein has been identified as a target of auto-antibodies in the autoimmune skin blistering disease pemphigus foliaceus. Disruption of this gene has also been associated with the skin diseases palmoplantar keratoderma and erythroderma. [provided by RefSeq, Feb 2015]
Database
DATA IMAGES
![Anti-Desmoglein 1 antibody [DSG1/1733] used in IHC (Paraffin sections) (IHC-P). GTX34675](/upload/website/prouct_img/middle/GTX34675/GTX34675_20170712_IHC-P_18121410_563.jpg "Anti-Desmoglein 1 antibody [DSG1/1733] used in IHC (Paraffin sections) (IHC-P). GTX34675")
|
GTX34675 IHC-P Image
Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-1 Monoclonal Antibody (DSG1/1733).
|
REFERENCE
There are currently no references for Desmoglein 1 antibody [DSG1/1733] (GTX34675). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Desmoglein 1 antibody [DSG1/1733] (GTX34675). Be the first to share your experience with this product.
![Anti-Desmoglein 1 antibody [DSG1/1733] used in IHC (Paraffin sections) (IHC-P). GTX34675](/upload/website/prouct_img/small/GTX34675/GTX34675_20170712_IHC-P_18121410_563.jpg "Anti-Desmoglein 1 antibody [DSG1/1733] used in IHC (Paraffin sections) (IHC-P). GTX34675")
![Anti-Desmoglein 1 antibody [DSG1/1733] used in IHC (Paraffin sections) (IHC-P). GTX34675](/upload/website/prouct_img/normal/GTX34675/GTX34675_20170712_IHC-P_18121410_563.jpg "Anti-Desmoglein 1 antibody [DSG1/1733] used in IHC (Paraffin sections) (IHC-P). GTX34675")
