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Desmoglein 1 antibody [DSG1/1733]

Anti-Desmoglein 1 antibody [DSG1/1733] used in IHC (Paraffin sections) (IHC-P). GTX34675

Cat No. GTX34675

Host

Mouse

Clonality

Monoclonal

Clone Name

DSG1/1733

Isotype

IgG1

Application

ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of ~150kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human desmoglein-1 protein fragment corresponding to intracellular domain (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Desmoglein 1 , Cdhf4 , Dg1 , Dsg , Epkhe , Epkhia , Ppks1 , Sppk1 , Dsg1

Background

This gene encodes a member of the desmoglein protein subfamily. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmoglein family members on chromosome 18. The encoded protein has been identified as a target of auto-antibodies in the autoimmune skin blistering disease pemphigus foliaceus. Disruption of this gene has also been associated with the skin diseases palmoplantar keratoderma and erythroderma. [provided by RefSeq, Feb 2015]

Database

DATA IMAGES

Anti-Desmoglein 1 antibody [DSG1/1733] used in IHC (Paraffin sections) (IHC-P). GTX34675

GTX34675 IHC-P Image

Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-1 Monoclonal Antibody (DSG1/1733).

REFERENCE

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REVIEW

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Package List Price ($)
$ 429