GeneTex
United States (US)

Desmoglein 2 antibody [N1N2], N-term

GTX102508_40114_IFA_19011506_368.jpg
GTX102508_IHC_19011506_742.jpg
GTX102508_40114_20170817_IHC-P_M_19011506_622.jpg
GTX102508_WB_19011506_491.jpg
GTX102508_40114_20171020_WB_19011506_156.jpg

Cat No. GTX102508

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

HeLa , 293T , H1299 , Molt-4

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Desmoglein 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Desmoglein 2,Cdhf5,Hdgc,Dsg2

Cellular Localization

Cell membrane , Cell junction , desmosome

Background

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102508_40114_IFA_19011506_368.jpg

GTX102508 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Desmoglein 2(GTX102508) antibody at 1:500 dilution.

GTX102508_IHC_19011506_742.jpg

GTX102508 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DU145 xenograft , using Desmoglein-2(GTX102508) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102508_40114_20170817_IHC-P_M_19011506_622.jpg

GTX102508 IHC-P Image

Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane and cytoplasm in mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102508_WB_19011506_491.jpg

GTX102508 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: H1299
C: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX102508 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102508_40114_20171020_WB_19011506_156.jpg

GTX102508 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169