GeneTex
  • Country / Location Selection

United States (US)

Desmoglein 3 antibody [5G11]

Anti-Desmoglein 3 antibody [5G11] used in IHC (Paraffin sections) (IHC-P). GTX34676

Cat No. GTX34676

Host

Mouse

Clonality

Monoclonal

Clone Name

5G11

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0ug/ml
ICC/IF 1-2ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human DSG3 extracellular protein fragment (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Desmoglein 3 , Cdhf6 , Pva , Dsg3

Background

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Desmoglein 3 antibody [5G11] used in IHC (Paraffin sections) (IHC-P). GTX34676

GTX34676 IHC-P Image

Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-3 Monoclonal Antibody (5G11).

REFERENCE

There are currently no references for Desmoglein 3 antibody [5G11] (GTX34676). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Desmoglein 3 antibody [5G11] (GTX34676). Be the first to share your experience with this product.
Package List Price ($)
$ 429