GeneTex
United States (US)

Desmoglein 3 antibody [DSG3/1535]

GTX34677_20170712_IHC-P_18121410_987.jpg
GTX34677_20170712_IHC-P_1_18121410_170.jpg

Cat No. GTX34677

Host Mouse
Clonality Monoclonal
Clone Name DSG3/1535
Isotype IgG1
Application IHC-P
Reactivity Human
APPLICATION

Application Note

IHC-P:1-2ug/ml for 30 minutes at RT.

Calculated MW

108 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (>80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human DSG3 extracellular protein fragment (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Desmoglein 3,Cdhf6,Pva,Dsg3

Background

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34677_20170712_IHC-P_18121410_987.jpg

GTX34677 IHC-P Image

Formalin-paraffin Human Lung Squamous Cell Carcinoma stained with Desmoglein-3 Monoclonal Antibody (DSG3/1535).

GTX34677_20170712_IHC-P_1_18121410_170.jpg

GTX34677 IHC-P Image

Formalin-fixed, paraffin-embedded Human Skin stained with Desmoglein-3 Monoclonal Antibody (DSG3/1535).

Package List Price ($)
$ 329