APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
A431
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.38 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Extracellular domain of human DSG3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886130
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
desmoglein 3 , CDHF6 , PVA
Cellular Localization
Cell membrane; Single-pass type I membrane protein , Cell junction , desmosome , Cell membrane , Cell junction , desmosome
Background
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX129931 IP Image
Immunoprecipitation of DSG3 protein from A431 whole cell extracts using 5 μg of DSG3 antibody (GTX129931).
Western blot analysis was performed using DSG3 antibody (GTX129931).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX129931 WB Image
DSG3 antibody detects DSG3 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DSG3 antibody (GTX129931) at a dilution of 1:1000.
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REFERENCE
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REVIEW
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