GeneTex
  • Country / Location Selection

United States (US)

Desmoglein 3 antibody

Anti-Desmoglein 3 antibody used in Immunoprecipitation (IP). GTX129931
Anti-Desmoglein 3 antibody used in Western Blot (WB). GTX129931

Cat No. GTX129931

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

A431

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Desmoglein 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

desmoglein 3 , CDHF6 , PVA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Cell junction , desmosome , Cell membrane , Cell junction , desmosome

Background

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Desmoglein 3 antibody used in Immunoprecipitation (IP). GTX129931

GTX129931 IP Image

Immunoprecipitation of DSG3 protein from A431 whole cell extracts using 5 μg of DSG3 antibody (GTX129931).
Western blot analysis was performed using DSG3 antibody (GTX129931).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Desmoglein 3 antibody used in Western Blot (WB). GTX129931

GTX129931 WB Image

DSG3 antibody detects DSG3 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DSG3 antibody (GTX129931) at a dilution of 1:1000.

REFERENCE

There are currently no references for Desmoglein 3 antibody (GTX129931). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Desmoglein 3 antibody (GTX129931). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169