APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , A549 , H1299 , HCT116 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.39 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Destrin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10620557
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
destrin, actin depolymerizing factor , ACTDP , ADF , HEL32 , bA462D18.2
Background
The product of this gene belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX114170 WB Image
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE GTX114170 diluted at 1:2000
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GTX114170 WB Image
Destrin antibody detects Destrin protein by Western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract C. 30 μg HCT116 whole cell lysate/extract 12 % SDS-PAGE Destrin antibody (GTX114170) dilution: 1:500
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GTX114170 WB Image
Destrin antibody detects Destrin protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 12 % SDS-PAGE Destrin antibody (GTX114170) dilution: 1:500
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REFERENCE
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REVIEW
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