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Diablo antibody [AT19F2]

Anti-Diablo antibody [AT19F2] used in Western Blot (WB). GTX57593
Anti-Diablo antibody [AT19F2] used in Western Blot (WB). GTX57593
Anti-Diablo antibody [AT19F2] used in Flow cytometry (FACS). GTX57593

Cat. No. GTX57593

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT19F2

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all Diablo products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Recommended starting dilution is 1:3000.
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Observed MW

21 kDa. The observed M.W. is based on the following publication. PMID: 29444163

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT19F2 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SMAC/Diablo protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

diablo IAP-binding mitochondrial protein , DFNA64 , DIABLO , SMAC , diablo IAPbinding mitochondrial protein , Smac , Diablo

Cellular Localization

Mitochondrion

Background

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-Diablo antibody [AT19F2] used in Western Blot (WB). GTX57593

GTX57593 WB Image

WB analysis of various samples using GTX57593 Diablo antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : Hep3B whole cell lysate
Lane 4 : MCF-7 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-Diablo antibody [AT19F2] used in Western Blot (WB). GTX57593

GTX57593 WB Image

WB analysis of Jurkat whole cell lysate using GTX57593 Diablo antibody.
Loading : 40 μg
Dilution : 1:3000

Anti-Diablo antibody [AT19F2] used in Flow cytometry (FACS). GTX57593

GTX57593 FACS Image

FACS analysis of HeLa cells using GTX57593 Diablo antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399