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Diablo antibody, C-term

Anti-Diablo antibody, C-term used in Western Blot (WB). GTX77790
Anti-Diablo antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77790

Cat. No. GTX77790

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Diablo products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Jurkat

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human Diablo

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_424738

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

diablo IAP-binding mitochondrial protein , DFNA64 , SMAC

Cellular Localization

Mitochondrion

Background

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-Diablo antibody, C-term used in Western Blot (WB). GTX77790

GTX77790 WB Image

WB analysis of Jurkat cells using GTX77790 Diablo antibody at 0.3125μg/ml.
Lane A : marker
Lane B : Jurkat cells

Anti-Diablo antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77790

GTX77790 IHC-P Image

IHC-P analysis of human heart tissue using GTX77790 Diablo antibody at 1:100. Left to right:DAPI, DIABLO, Merge. The low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399