GeneTex
United States (US)

Diablo antibody [N1C3]

GTX106746_40051_IHC_19011506_471.jpg
GTX106746_WB_19011506_649.jpg
GTX106746_40051_20160114_WB_M_testis_19011506_344.jpg

Cat No. GTX106746

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse testis

Predict Reactivity

Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Diablo. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DIABLO-S antibody, FLJ10537 antibody, FLJ25049 antibody, SMAC antibody, SMAC3 antibody, DIABLO antibody, second mitochondria-derived activator of caspase antibody, mitochondrial Smac protein antibody, 0610041G12Rik antibody, direct IAP-binding protein with low pI antibody, "diablo homolog, mitochondrial antibody", diablo homolog (Drosophila) antibody

Cellular Localization

Mitochondrion

Background

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106746_40051_IHC_19011506_471.jpg

GTX106746 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Diablo(GTX106746) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106746_WB_19011506_649.jpg

GTX106746 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX106746 diluted at 1:1000

GTX106746_40051_20160114_WB_M_testis_19011506_344.jpg

GTX106746 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Diablo antibody [N1C3] (GTX106746) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169