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Diablo antibody [SMAC 17 1-87]

Anti-Diablo antibody [SMAC 17 1-87] used in Western Blot (WB). GTX15827
Anti-Diablo antibody [SMAC 17 1-87] used in IHC (Paraffin sections) (IHC-P). GTX15827
Anti-Diablo antibody [SMAC 17 1-87] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15827
Anti-Diablo antibody [SMAC 17 1-87] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15827
Anti-Diablo antibody [SMAC 17 1-87] used in IHC (Paraffin sections) (IHC-P). GTX15827
Anti-Diablo antibody [SMAC 17 1-87] used in IHC (Paraffin sections) (IHC-P). GTX15827
Anti-Diablo antibody [SMAC 17 1-87] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15827

Cat. No. GTX15827

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SMAC 17 1-87

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Diablo products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:20-1:200
IHC-P 1:20-1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

C-Terminal His tagged recombinant human Smac/DIABLO.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

diablo IAP-binding mitochondrial protein , DFNA64 , SMAC

Cellular Localization

Mitochondrion

Background

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-Diablo antibody [SMAC 17 1-87] used in Western Blot (WB). GTX15827

GTX15827 WB Image

WB analysis of 25 ug of 293 (Lane 1), Hela (Lane 2), and PC12 cell lysates (Lane 3) using GTX15827 Diablo antibody [SMAC 17 1-87].
Dilution : 1:1000

Anti-Diablo antibody [SMAC 17 1-87] used in IHC (Paraffin sections) (IHC-P). GTX15827

GTX15827 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX15827 Diablo antibody [SMAC 17 1-87].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-Diablo antibody [SMAC 17 1-87] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15827

GTX15827 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX15827 Diablo antibody [SMAC 17 1-87]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-Diablo antibody [SMAC 17 1-87] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15827

GTX15827 ICC/IF Image

ICC/IF analysis of A431 cells using GTX15827 Diablo antibody [SMAC 17 1-87]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-Diablo antibody [SMAC 17 1-87] used in IHC (Paraffin sections) (IHC-P). GTX15827

GTX15827 IHC-P Image

IHC-P analysis of human testis tissue using GTX15827 Diablo antibody [SMAC 17 1-87].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-Diablo antibody [SMAC 17 1-87] used in IHC (Paraffin sections) (IHC-P). GTX15827

GTX15827 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX15827 Diablo antibody [SMAC 17 1-87].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-Diablo antibody [SMAC 17 1-87] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15827

GTX15827 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX15827 Diablo antibody [SMAC 17 1-87]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399