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Diablo antibody

Anti-Diablo antibody used in IHC (Paraffin sections) (IHC-P). GTX23930
Anti-Diablo antibody used in Western Blot (WB). GTX23930

Cat. No. GTX23930

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($479)
See all Diablo products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1μg/ml
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to aa 225-239 of human Smac (accession no. AAF87716).

Purification

Purified by DEAE chromatography

Conjugation

Unconjugated

RRID

AB_374235

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DFNA64 , DIABLO , SMAC , diablo IAPbinding mitochondrial protein , Smac , Diablo , diablo IAP-binding mitochondrial protein

Cellular Localization

Mitochondrion

Background

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-Diablo antibody used in IHC (Paraffin sections) (IHC-P). GTX23930

GTX23930 IHC-P Image

Immunohistochemical staining of Smac in human ovary with Diablo antibody at 5μg /ml.

Anti-Diablo antibody used in Western Blot (WB). GTX23930

GTX23930 WB Image

Western blot analysis of Smac in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide with Diablo antibody at 1μg/ml.
Positive control: Human heart tissue lysate.

REFERENCE

Application Reference

Tian J et al. PLoS One 2010; 5 (12) : e14218 Expression of baculovirus anti-apoptotic genes p35 and op-iap in cotton (Gossypium hirsutum L.) enhances tolerance to verticillium wilt.
Application : WB
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SDS
PBS.pdf
Package List Price ($)
$ 479