APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431
Predict Reactivity
Mouse, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Dicer. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888389
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dicer 1, ribonuclease III , DCR1 , Dicer , Dicer1e , GLOW , HERNA , K12H4.8-LIKE , MNG1 , RMSE2
Cellular Localization
Cytoplasm
Background
This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Dicer antibody [GT1176] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633917](/templates/default/js/lightbox/images/loading.gif "Anti-Dicer antibody [GT1176] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633917")
|
GTX633917 ICC/IF Image
Dicer antibody [GT1176] detects Dicer protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Dicer stained by Dicer antibody [GT1176] (GTX633917) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10μm.
|
|
|
GTX633917 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [GT1176] (GTX633917) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for Dicer antibody [GT1176] (GTX633917). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Dicer antibody [GT1176] (GTX633917). Be the first to share your experience with this product.
![Anti-Dicer antibody [GT1176] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633917](/upload/website/prouct_img/small/GTX633917/GTX633917_42814_20180321_ICC_IF_w_23061202_996.webp "Anti-Dicer antibody [GT1176] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633917")
![Anti-Dicer antibody [GT1176] used in Western Blot (WB). GTX633917](/upload/website/prouct_img/small/GTX633917/GTX633917_42814_20160420_WB_w_23061202_443.webp "Anti-Dicer antibody [GT1176] used in Western Blot (WB). GTX633917")
![Anti-Dicer antibody [GT1176] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633917](/upload/website/prouct_img/normal/GTX633917/GTX633917_42814_20180321_ICC_IF_w_23061202_996.webp "Anti-Dicer antibody [GT1176] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633917")
![Anti-Dicer antibody [GT1176] used in Western Blot (WB). GTX633917](/upload/website/prouct_img/normal/GTX633917/GTX633917_42814_20160420_WB_w_23061202_443.webp "Anti-Dicer antibody [GT1176] used in Western Blot (WB). GTX633917")