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Dicer antibody [HL1232]

Recombinant antibody KO/KD
Anti-Dicer antibody [HL1232] used in Western Blot (WB). GTX636580
Anti-Dicer antibody [HL1232] used in Western Blot (WB). GTX636580
Anti-Dicer antibody [HL1232] used in IHC (Paraffin sections) (IHC-P). GTX636580
Anti-Dicer antibody [HL1232] used in Western Blot (WB). GTX636580
Anti-Dicer antibody [HL1232] used in Western Blot (WB). GTX636580
Anti-Dicer antibody [HL1232] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636580
Anti-Dicer antibody [HL1232] used in Western Blot (WB). GTX636580

Cat. No. GTX636580

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1232

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Dicer products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

219 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dicer. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910014

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dicer 1, ribonuclease III , DCR1 , Dicer , Dicer1e , GLOW , HERNA , K12H4.8-LIKE , MNG1 , RMSE2

Cellular Localization

Cytoplasm

Background

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES

Anti-Dicer antibody [HL1232] used in Western Blot (WB). GTX636580

GTX636580 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dicer antibody [HL1232] used in Western Blot (WB). GTX636580

GTX636580 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dicer antibody [HL1232] used in IHC (Paraffin sections) (IHC-P). GTX636580

GTX636580 IHC-P Image

Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Dicer stained by Dicer antibody [HL1232] (GTX636580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dicer antibody [HL1232] used in Western Blot (WB). GTX636580

GTX636580 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Dicer antibody [HL1232] used in Western Blot (WB). GTX636580

GTX636580 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Dicer antibody [HL1232] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636580

GTX636580 ICC/IF Image

Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dicer stained by Dicer antibody [HL1232] (GTX636580) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Dicer antibody [HL1232] used in Western Blot (WB). GTX636580

GTX636580 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations