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Dicer antibody

KO/KD
Anti-Dicer antibody used in Western Blot (WB). GTX130536
Anti-Dicer antibody used in Western Blot (WB). GTX130536
Anti-Dicer antibody used in Western Blot (WB). GTX130536
Anti-Dicer antibody used in Western Blot (WB). GTX130536

Cat. No. GTX130536

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Dicer products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

219 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , MCF-7 , SW480

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dicer. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886300

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dicer 1, ribonuclease III , DCR1 , Dicer , Dicer1e , GLOW , HERNA , K12H4.8-LIKE , MNG1 , RMSE2

Cellular Localization

Cytoplasm

Background

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Dicer antibody used in Western Blot (WB). GTX130536

GTX130536 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody (GTX130536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dicer antibody used in Western Blot (WB). GTX130536

GTX130536 WB Image

KO/KD

Non-transfected (–) and transfected (+) SW480 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody (GTX130536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dicer antibody used in Western Blot (WB). GTX130536

GTX130536 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody (GTX130536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dicer antibody used in Western Blot (WB). GTX130536

GTX130536 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody (GTX130536) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

X Lin et al. Neurotoxicology 2023; 100 : 85-99 Cobalt-induced apoptosis of cochlear organotypic cultures and HEI-OC1 cells is mediated by Dicer
Application : WB
Jie-Ning Li et al. Front Cell Dev Biol 2021; 9 : 671244 E-cadherin Interacts With Posttranslationally-Modified AGO2 to Enhance miRISC Activity.
Application : WB
Reactivity : Human
Davide P et al. Cell Mol Gastroenterol Hepatol 2015; 1 (6) : 646-663 Lipid-Induced Hepatocyte-Derived Extracellular Vesicles Regulate Hepatic Stellate Cells via MicroRNA Targeting Peroxisome Proliferator-Activated Receptor γ.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169