Identification of Dis2 phosphorylated at T316 by western blotting with the antibody. S. pombe crude extracts prepared from mitotic and G2-phase were analysed by WB. Dis2 and phosphorylated Dis2 were detected with anti-Dis2 antibody and this antibody (GTX64105), respectively. Phosphorylation is increased in mitosis and sensitive to λ-phosphatase. The antibody was used at 1/1,000 dilution in PBS containing 0.1% Tween and 1% milk. Courtesy of Mr M. Swaffer at Cancer Research UK