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Dis2 (phospho Thr316) (S. pombe) antibody

Anti-Dis2 (phospho Thr316) (S. pombe) antibody used in Western Blot (WB). GTX64105

Cat. No. GTX64105

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Schizosaccharomyces pombe
Package
50 μl ($399)

APPLICATION

Application Note

Western blotting (1:1,000-1:2,000 dilution).

Calculated MW

38 kDa. ( Note )

Product Note

Reacts with S. pombe Dis2 protein phosphorylated at Thr316. Not tested with other species

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Yeast

Immunogen

Synthetic peptide NWHMT(PO3)PPRKN conjugated to KLF

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bws1 , sds1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-Dis2 (phospho Thr316) (S. pombe) antibody used in Western Blot (WB). GTX64105

GTX64105 WB Image

Identification of Dis2 phosphorylated at T316 by western blotting with the antibody. S. pombe crude extracts prepared from mitotic and G2-phase were analysed by WB. Dis2 and phosphorylated Dis2 were detected with anti-Dis2 antibody and this antibody (GTX64105), respectively. Phosphorylation is increased in mitosis and sensitive to λ-phosphatase. The antibody was used at 1/1,000 dilution in PBS containing 0.1% Tween and 1% milk. Courtesy of Mr M. Swaffer at Cancer Research UK

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399