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Dishevelled 2 antibody [N2C2], Internal

Orthogonal
Anti-Dishevelled 2 antibody [N2C2], Internal used in Western Blot (WB). GTX103878
Anti-Dishevelled 2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103878
Anti-Dishevelled 2 antibody [N2C2], Internal used in Western Blot (WB). GTX103878
Anti-Dishevelled 2 antibody [N2C2], Internal used in Immunohistochemistry (IHC). GTX103878
Anti-Dishevelled 2 antibody [N2C2], Internal used in Western Blot (WB). GTX103878
Anti-Dishevelled 2 antibody [N2C2], Internal used in IHC (Frozen sections) (IHC-Fr). GTX103878

Cat. No. GTX103878

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Dishevelled 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

Molt-4 , Raji , 293T

Predict Reactivity

Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dishevelled 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950156

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dishevelled segment polarity protein 2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Dishevelled 2 antibody [N2C2], Internal used in Western Blot (WB). GTX103878

GTX103878 WB Image

Sample(30 μg whole cell lysate)
A: MOLT4 (GTX27912)
B: Raji (GTX27908)
7.5% SDS PAGE
GTX103878 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dishevelled 2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103878

GTX103878 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung SCC, using Dishevelled 2(GTX103878) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dishevelled 2 antibody [N2C2], Internal used in Western Blot (WB). GTX103878

GTX103878 WB Image

Whole cell extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Dishevelled 2 antibody [N2C2], Internal (GTX103878) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Dishevelled 2 antibody [N2C2], Internal used in Immunohistochemistry (IHC). GTX103878

GTX103878 IHC Image

cytoplasm in the photoreceptor
Immunohistochemistry of flat-mounted zebrafish retina using an antibody against Dishevelled 2 antibody [N2C2], Internal (GTX103878) at a 1:200 dilution.

Anti-Dishevelled 2 antibody [N2C2], Internal used in Western Blot (WB). GTX103878

GTX103878 WB Image

Orthogonal

The data was published in the 2020 in Front Cell Dev Biol. PMID: 33015048

Anti-Dishevelled 2 antibody [N2C2], Internal used in IHC (Frozen sections) (IHC-Fr). GTX103878

GTX103878 IHC-Fr Image

The data was published in the journal PLoS One in 2013. PMID: 23437169

REFERENCE

Application Reference

Khan K et al. Front Cell Dev Biol 2020; 8 : 862 The Role of Wnt/β-Catenin Pathway Mediators in Aortic Valve Stenosis.
Application : WB, IHC-P
Reactivity : Human
Rodriguez-Gil DJ et al. PLoS One 2013; 8 (2) : e56561 Dishevelled proteins are associated with olfactory sensory neuron presynaptic terminals.
Application : IHC
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169