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Dishevelled 2 antibody [N2C2], Internal

Anti-Dishevelled 2 antibody [N2C2], Internal used in Western Blot (WB). GTX103878
Anti-Dishevelled 2 antibody [N2C2], Internal used in Western Blot (WB). GTX103878
Anti-Dishevelled 2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103878

Cat No. GTX103878

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dishevelled 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

dishevelled segment polarity protein 2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Dishevelled 2 antibody [N2C2], Internal used in Western Blot (WB). GTX103878

GTX103878 WB Image

Whole cell extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Dishevelled 2 antibody [N2C2], Internal (GTX103878) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Dishevelled 2 antibody [N2C2], Internal used in Western Blot (WB). GTX103878

GTX103878 WB Image

Sample(30 µg whole cell lysate)
A: MOLT4 (GTX27912)
B: Raji (GTX27908)
7.5% SDS PAGE
GTX103878 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dishevelled 2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103878

GTX103878 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung SCC, using Dishevelled 2(GTX103878) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169