GeneTex
United States (US)

Dishevelled 3 antibody [N1N2], N-term

GTX102509_WB_19011506_677.jpg
GTX102509_39728_20141114_IHC_M_19011506_427.jpg
GTX102509_39728_20150410_IFA_19011506_946.jpg

Cat No. GTX102509

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

HCT116
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Dishevelled 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dishevelled Segment Polarity Protein 3,Drs3,Dvl3

Cellular Localization

Cytoplasm

Background

This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102509_WB_19011506_677.jpg

GTX102509 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116 7.5% SDS PAGE
GTX102509 diluted at 1:10000

GTX102509_39728_20141114_IHC_M_19011506_427.jpg

GTX102509 IHC-P Image

Dishevelled 3 antibody [N1N2], N-term detects Dishevelled 3 protein at cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Dishevelled 3 antibody [N1N2], N-term (GTX102509) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102509_39728_20150410_IFA_19011506_946.jpg

GTX102509 ICC/IF Image

Dishevelled 3 antibody [N1N2], N-term detects Dishevelled 3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dishevelled 3 protein stained by Dishevelled 3 antibody [N1N2], N-term (GTX102509) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169