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Dishevelled 3 antibody [N1N2], N-term

Anti-Dishevelled 3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102509
Anti-Dishevelled 3 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102509
Anti-Dishevelled 3 antibody [N1N2], N-term used in Western Blot (WB). GTX102509
Anti-Dishevelled 3 antibody [N1N2], N-term used in Immunohistochemistry (IHC). GTX102509
Anti-Dishevelled 3 antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX102509

Cat. No. GTX102509

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, IHC

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Dishevelled 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

HCT116

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Dishevelled 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240709

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dishevelled segment polarity protein 3 , DRS3

Cellular Localization

Cytoplasm

Background

This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Dishevelled 3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102509

GTX102509 IHC-P Image

Dishevelled 3 antibody [N1N2], N-term detects Dishevelled 3 protein at cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Dishevelled 3 antibody [N1N2], N-term (GTX102509) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Dishevelled 3 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102509

GTX102509 ICC/IF Image

Dishevelled 3 antibody [N1N2], N-term detects Dishevelled 3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dishevelled 3 protein stained by Dishevelled 3 antibody [N1N2], N-term (GTX102509) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Dishevelled 3 antibody [N1N2], N-term used in Western Blot (WB). GTX102509

GTX102509 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116 7.5% SDS PAGE
GTX102509 diluted at 1:10000

Anti-Dishevelled 3 antibody [N1N2], N-term used in Immunohistochemistry (IHC). GTX102509

GTX102509 IHC Image

cytoplasm in the photoreceptor
Immunohistochemistry of flat-mounted zebrafish retina using an antibody against Dishevelled 3 antibody [N1N2], N-term (GTX102509) at a 1:200 dilution.

Anti-Dishevelled 3 antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX102509

GTX102509 IHC-Fr Image

The data was published in the journal PLoS One in 2013. PMID: 23437169

REFERENCE

Application Reference

Pai VC et al. Oncogene 2018; (Epub) ASPM promotes prostate cancer stemness and progression by augmenting Wnt-Dvl-3-β-catenin signaling.
Application : WB, IP
Reactivity : Human
Wang J et al. Clin Cancer Res 2013; 19 (20) : 5580-90 Integrative genomics analysis identifies candidate drivers at 3q26-29 amplicon in squamous cell carcinoma of the lung.
Rodriguez-Gil DJ et al. PLoS One 2013; 8 (2) : e56561 Dishevelled proteins are associated with olfactory sensory neuron presynaptic terminals.
Application : IHC
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169