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Dog IL6 protein (active)

Dog IL6 protein (active). GTX00077-pro
Dog IL6 protein (active). GTX00077-pro
Dog IL6 protein (active). GTX00077-pro

Cat No. GTX00077-pro

Application Functional Assay
Species Dog
Package
10 μg ($329)
APPLICATION

Application Note

Cell proliferation of Raji cells after stimulated with IL6. Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with 1% serum standard RPMI-1640 which contains various concentrations of recombinant dog IL6. After incubated for 5 days,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of Raji cells after incubation with IL6 for 5 days observed by inverted microscope.

Observed MW

25 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala18~Met207 (NP_001003301.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 6,IL-6

Database

Research Area

DATA IMAGES
Dog IL6 protein (active). GTX00077-pro

GTX00077-pro Functional Assay Image

Cell proliferation effect of GTX00077-pro Dog IL6 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated Raji cells cultured in RPMI-1640 for 5 days.
(B) Raji cells cultured in RPMI-1640, stimulated with 1 ng/ml IL6 for 5 days.

Dog IL6 protein (active). GTX00077-pro

GTX00077-pro Image

WB analysis of GTX00077-pro Dog IL6 protein (active).

Dog IL6 protein (active). GTX00077-pro

GTX00077-pro Image

SDS-PAGE analysis of GTX00077-pro Dog IL6 protein (active).

REFERENCE
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Package List Price ($)
$ 329