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Donkey Anti-Goat IgG antibody, F(ab')2 fragment, pre-adsorbed (PE)

Donkey Anti-Goat IgG antibody, F(ab')2 fragment, pre-adsorbed (PE). GTX27004
Donkey Anti-Goat IgG antibody, F(ab')2 fragment, pre-adsorbed (PE). GTX27004

Cat. No. GTX27004

Host

Donkey

Clonality

Polyclonal

Isotype

IgG F(ab')2

Application

WB, ICC/IF, FACS, Dot, ELISA

Reactivity

Goat
Package
500 μl ($259)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:250
FACS 1:100-1:250
Dot Assay dependent
ELISA Assay dependent
Not tested in other applications.

Product Note

Pre-adsorbed with Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat serum proteins. May react with immunoglobulins from other species.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Goat

Immunogen

Goat IgG whole molecule

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities, pepsin digestion, and chromatographic separation.
From serum

Conjugation

Phycoerythrin (PE)
Ratio : 4.74 molecules PE per Donkey IgG F(ab')2 molecule.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Donkey Anti-Goat IgG antibody, F(ab')2 fragment, pre-adsorbed (PE). GTX27004

GTX27004 Dot Image

Dot blot analysis of goat IgG using GTX27004 Donkey Anti-Goat IgG antibody, F(ab')2 fragment, pre-adsorbed (PE).
Lane 1 : 100 ng
Lane 2 : 33.3 ng
Lane 3 : 11.1 ng
Lane 4 : 3.7 ng
Lane 5 : 1.23 ng
Dilution : 1 μg/mL

Donkey Anti-Goat IgG antibody, F(ab')2 fragment, pre-adsorbed (PE). GTX27004

GTX27004 Dot Image

Dot blot analysis of goat IgG using GTX27004 Donkey Anti-Goat IgG antibody, F(ab')2 fragment, pre-adsorbed (PE).
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 259