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United States (US)

Donkey Anti-Rabbit IgG antibody (Rhodamine)

Cat. No. GTX26799

Reference 2

Host

 Donkey

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, Dot, ELISA

Reactivity

 Rabbit
Package
1 mg ($149)
See all Rabbit IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:1000-1:5000
FACS 1:500-1:2500
Dot Assay dependent
ELISA 1:10000-1:50000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Rabbit IgG whole molecule

Target Isotype

IgG

Purification

IgG fraction
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities.

Conjugation

Rhodamine
Ratio : 2.4 molecules Rhodamine per Donkey IgG molecule.

RRID

AB_378891

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

REFERENCE

Application Reference

Lee JG et al. J Biol Chem 2018; 293 (10) : 3758-3769 Fibroblast growth factor 2 induces proliferation and fibrosis via SNAI1-mediated activation of CDK2 and ZEB1 in corneal endothelium.
Lee JG et al. J Biol Chem 2015; 290 (44) : 26752-64 WNT10B enhances proliferation through β-catenin and RAC1 GTPase in human corneal endothelial cells.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 149