GeneTex
United States (US)

Dopamine Receptor D1 antibody [C2C3], C-term

GTX100354_39609_20150923_IHC-Fr_M_19011506_411.jpg
GTX100354_39609_20181227_ICC_IF_R_19022001_313.jpg
GTX100354_39609_IHC_19011506_576.jpg
GTX100354_WB_19011506_491.jpg

Cat No. GTX100354

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Dopamine Receptor D1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dopamine Receptor D1,Dadr,Drd1A,Drd1

Cellular Localization

Cell membrane; Multi-pass membrane protein , Endoplasmic reticulum membrane; Multi-pass membrane protein , Cell membrane , Endoplasmic reticulum membrane

Background

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100354_39609_20150923_IHC-Fr_M_19011506_411.jpg

GTX100354 IHC-Fr Image

Dopamine receptor D1 antibody detects dopamine receptor D1 protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: dopamine receptor D1 antibody [GTX100354] diluted at 1:500. Blue: DAPI

GTX100354_39609_20181227_ICC_IF_R_19022001_313.jpg

GTX100354 ICC/IF Image

Dopamine Receptor D1 antibody [C2C3], C-term detects Dopamine Receptor D1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [C2C3], C-term (GTX100354) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100354_39609_IHC_19011506_576.jpg

GTX100354 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Dopamine Receptor D1(GTX100354) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100354_WB_19011506_491.jpg

GTX100354 WB Image

Sample (30 µg of whole cell lysate)
A: IMR32 10% SDS PAGE
GTX100354 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169