Dopamine Receptor D1 antibody [C2C3], C-term

Name: Dopamine Receptor D1 antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX100354
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Dopamine Receptor D1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100354

Anti-Dopamine Receptor D1 antibody [C2C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX100354

Anti-Dopamine Receptor D1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100354

Anti-Dopamine Receptor D1 antibody [C2C3], C-term used in Western Blot (WB). GTX100354

Cat. No. GTX100354

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Dopamine Receptor D1 products

View product citations for antibody GTX100354 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Dopamine Receptor D1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1240724

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dopamine receptor D1 , DADR , DRD1A

Cellular Localization

Cell membrane; Multi-pass membrane protein , Endoplasmic reticulum membrane; Multi-pass membrane protein , Cell membrane , Endoplasmic reticulum membrane

Background

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq]

Database

Gene ID: 1812 DRD1
UniProt: P21728 DRD1

Research Area

DATA IMAGES

GTX100354 ICC/IF Image

Dopamine Receptor D1 antibody [C2C3], C-term detects Dopamine Receptor D1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [C2C3], C-term (GTX100354) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100354 IHC-Fr Image

Dopamine Receptor D1 antibody [C2C3], C-term detects Dopamine Receptor D1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebral cortex.
Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [C2C3], C-term (GTX100354) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100354 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Dopamine Receptor D1(GTX100354) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100354 WB Image

Sample (30 μg of whole cell lysate)
A: IMR32 10% SDS PAGE
GTX100354 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hongzhu Li et al. Int J Mol Med 2022; 49 (1) The DR1?CSE/H₂S system inhibits renal fibrosis by downregulating the ERK1/2 signaling pathway in diabetic mice.

Application : WB

Reactivity : Mouse

Tapia E et al. Free Radic Res 2016; 50 (7) : 781-92 Mycophenolate mofetil and curcumin provide comparable therapeutic benefit in experimental chronic kidney disease: role of Nrf2-Keap1 and renal * pathways.

Application : WB

Reactivity : Rat

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