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Dopamine Receptor D1 antibody [HL2680]

Recombinant antibody Orthogonal Overexpression
Anti-Dopamine Receptor D1 antibody [HL2680] used in Western Blot (WB). GTX639344
Anti-Dopamine Receptor D1 antibody [HL2680] used in Western Blot (WB). GTX639344

Cat. No. GTX639344

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2680

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Dopamine Receptor D1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Product Note

This antibody was raised against human Dopamine Receptor D1 Intracellular domain, and it does not cross react with human DRD2 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human Dopamine Receptor D1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dopamine receptor D1 , DADR , DRD1A

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Cell projection, dendrite,Cell projection, dendritic spine

Background

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Dopamine Receptor D1 antibody [HL2680] used in Western Blot (WB). GTX639344

GTX639344 WB Image

Overexpression

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D1 antibody [HL2680] (GTX639344) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dopamine Receptor D1 antibody [HL2680] used in Western Blot (WB). GTX639344

GTX639344 WB Image

Orthogonal

Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D1 antibody [HL2680] (GTX639344) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations