Dopamine Receptor D2 antibody [HL1478]

Name: Dopamine Receptor D2 antibody [HL1478]
Brand: GeneTex
SKU: GTX636952
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Dopamine Receptor D2 antibody [HL1478] used in IHC (Paraffin sections) (IHC-P). GTX636952

Anti-Dopamine Receptor D2 antibody [HL1478] used in Western Blot (WB). GTX636952

Anti-Dopamine Receptor D2 antibody [HL1478] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636952

Anti-Dopamine Receptor D2 antibody [HL1478] used in Functional Assay (Functional Assay). GTX636952

Cat. No. GTX636952

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1478
Isotype	IgG
Application	WB, ICC/IF, IHC-P, Functional Assay
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Dopamine Receptor D2 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent
Functional Assay	Assay dependent

Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-term region of Human Dopamine Receptor D2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dopamine receptor D2 , D2DR , D2R

Cellular Localization

Cell membrane

Background

This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1813 DRD2
UniProt: P14416 DRD2

Research Area

DATA IMAGES

GTX636952 IHC-P Image

Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636952 IHC-P Image

Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636952 WB Image

Unboiled mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody (Ab5084p) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX636952 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX636952 IHC-P Image

GTX636952 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX636952 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000 and competitor's antibody (Ab5084p) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX636952 ICC/IF Image

Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in ice-cold MeOH for 5 min.
Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

GTX636952 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636952 WB Image

Unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

GTX636952 Functional Assay Image

Dopamine Receptor D2 antibody [HL1478] (GTX636952) specifically detects DRD2 protein on a VirD^TM-GPCR array.

Yellow: DRD2
White: DRD3
Blue: DRD5
Bottom left: Positive control

GTX636952 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX636952 ICC/IF Image

Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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REVIEW