APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Dog, Pig, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-term region of Human Dopamine Receptor D2. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
dopamine receptor D2 , D2DR , D2R
Cellular Localization
Cell membrane
Background
This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX636952 WB Image
Unboiled mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody (Ab5084p) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636952 WB Image
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000 and competitor's antibody (Ab5084p) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636952 IHC-P Image
Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636952 WB Image
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636952 WB Image
Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636952 IHC-P Image
Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636952 WB Image
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636952 WB Image
Unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
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GTX636952 IHC-P Image
Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636952 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636952 ICC/IF Image
Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636952 ICC/IF Image
Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis.Sample: SH-SY5Y cells were fixed in ice-cold MeOH for 5 min.Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
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REFERENCE
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REVIEW
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