Dopamine Receptor D2 antibody [HL1478]

Dopamine Receptor D2 antibody [HL1478] (GTX636952) detects DRD2 protein by flow cytometry analysis.
Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer to permeabilize the cell membrane.
The cells were following stained with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) control at 4ºC for 1 hour.

Dopamine Receptor D2 antibody [HL1478] (GTX636952) specifically detects DRD2 protein on a VirD^TM-GPCR array.

Yellow: DRD2
White: DRD3
Blue: DRD5
Bottom left: Positive control

Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in ice-cold MeOH for 5 min.
Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Unboiled mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.