Dopamine Receptor D4 antibody [HL2850]

Dopamine Receptor D4 antibody [HL2850] (GTX640121) detects Dopamine Receptor D4 protein by flow cytometry analysis.
Sample: SK-N-AS and HeLa cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer.
The cells were following stained with Dopamine Receptor D4 antibody [HL2850] (GTX640121) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) control at 4ºC for 1 hour.
The classification of protein expression as positive or negative was primarily based on data from the Human Protein Atlas (HPA).

Dopamine Receptor D4 antibody [HL2850] (GTX640121) detects Dopamine Receptor D4 protein by flow cytometry analysis. Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Dopamine Receptor D4 antibody [HL2850] (GTX640121) diluted at 1:50 or a Rabbit monoclonal IgG isotype control at 4ºC for 1 hour.

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D4 antibody [HL2850] (GTX640121) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Dopamine Receptor D4 antibody [HL2850] detects Dopamine Receptor D4 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human stomach tumor.
Dopamine Receptor D4 stained by Dopamine Receptor D4 antibody [HL2850] (GTX640121) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min