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Dopamine Transporter antibody

Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152
Anti-Dopamine Transporter antibody used in IHC (Paraffin sections) (IHC-P). GTX133152
Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152
Anti-Dopamine Transporter antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133152
Anti-Dopamine Transporter antibody used in IHC (Paraffin sections) (IHC-P). GTX133152
Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152

Cat No. GTX133152

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Mouse, Rat, Zebrafish
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

rat olfactory bulb , mouse olfactory bulb

Predict Reactivity

Human, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Mouse Dopamine Transporter. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

solute carrier family 6 (neurotransmitter transporter, dopamine), member 3,DAT,Dat1

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.

Database

Research Area

DATA IMAGES
Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152

GTX133152 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dopamine Transporter antibody (GTX133152) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dopamine Transporter antibody used in IHC (Paraffin sections) (IHC-P). GTX133152

GTX133152 IHC-P Image

Dopamine Transporter antibody detects Dopamine Transporter protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse olfactory bulb.
Dopamine Transporter stained by Dopamine Transporter antibody (GTX133152) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152

GTX133152 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dopamine Transporter antibody (GTX133152) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dopamine Transporter antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133152

GTX133152 ICC/IF Image

Dopamine Transporter antibody detects Dopamine Transporter protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dopamine Transporter stained by Dopamine Transporter antibody (GTX133152) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Dopamine Transporter antibody used in IHC (Paraffin sections) (IHC-P). GTX133152

GTX133152 IHC-P Image

Dopamine Transporter antibody detects Dopamine Transporter protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse olfactory bulb.
Dopamine Transporter stained by Dopamine Transporter antibody (GTX133152) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152

GTX133152 WB Image

Various tissue extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dopamine Transporter antibody (GTX133152) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169