APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Dopamine beta Hydroxylase. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909986
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dopamine beta-hydroxylase , DBM , ORTHYP1
Cellular Localization
Secreted
Background
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Western Blot (WB). GTX636467](/templates/default/js/lightbox/images/loading.gif "Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Western Blot (WB). GTX636467")
|
GTX636467 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine beta Hydroxylase antibody [HL1163] (GTX636467) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.
|
|
|
GTX636467 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody [HL1163] (GTX636467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
|
|
GTX636467 ICC/IF Image
Dopamine beta Hydroxylase antibody [HL1163] detects Dopamine beta Hydroxylase protein at cytoplasm by immunofluorescent analysis.Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody [HL1163] (GTX636467) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
|
REFERENCE
There are currently no references for Dopamine beta Hydroxylase antibody [HL1163] (GTX636467). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Dopamine beta Hydroxylase antibody [HL1163] (GTX636467). Be the first to share your experience with this product.
![Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Western Blot (WB). GTX636467](/upload/website/prouct_img/small/GTX636467/GTX636467_44480_20220819_WB_competitor_watermark_22082402_433.webp "Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Western Blot (WB). GTX636467")
![Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Western Blot (WB). GTX636467](/upload/website/prouct_img/small/GTX636467/GTX636467_44480_20220819_WB_TPM_watermark_22082402_465.webp "Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Western Blot (WB). GTX636467")
![Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636467](/upload/website/prouct_img/small/GTX636467/GTX636467_44480_20230210_ICC_IF_23021401_517.webp "Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636467")
![Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Western Blot (WB). GTX636467](/upload/website/prouct_img/normal/GTX636467/GTX636467_44480_20220819_WB_competitor_watermark_22082402_433.webp "Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Western Blot (WB). GTX636467")
![Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Western Blot (WB). GTX636467](/upload/website/prouct_img/normal/GTX636467/GTX636467_44480_20220819_WB_TPM_watermark_22082402_465.webp "Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Western Blot (WB). GTX636467")
![Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636467](/upload/website/prouct_img/normal/GTX636467/GTX636467_44480_20230210_ICC_IF_23021401_517.webp "Anti-Dopamine beta Hydroxylase antibody [HL1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636467")