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Dopamine beta Hydroxylase antibody

Anti-Dopamine beta Hydroxylase antibody used in Western Blot (WB). GTX101616
Anti-Dopamine beta Hydroxylase antibody used in Western Blot (WB). GTX101616
Anti-Dopamine beta Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX101616
Anti-Dopamine beta Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX101616
Anti-Dopamine beta Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX101616

Cat No. GTX101616

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

SH-SY5Y

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dopamine beta Hydroxylase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dopamine Beta-Hydroxylase,Dbm,Dbh

Cellular Localization

Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle , secretory vesicle lumen , Cytoplasmic vesicle , secretory vesicle , chromaffin granule lumen , secretory vesicle membrane , chromaffin granule membrane

Background

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq]

Database

DATA IMAGES
Anti-Dopamine beta Hydroxylase antibody used in Western Blot (WB). GTX101616

GTX101616 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dopamine beta hydroxylase antibody (GTX101616) diluted at 1:2000 and competitor's antibody ( #8586S) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Dopamine beta Hydroxylase antibody used in Western Blot (WB). GTX101616

GTX101616 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody (GTX101616) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Dopamine beta Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX101616

GTX101616 IHC-P Image

Dopamine beta hydroxylase antibody detects DBH protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Dopamine beta hydroxylase antibody (GTX101616) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Dopamine beta Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX101616

GTX101616 IHC-P Image

Dopamine beta hydroxylase antibody detects DBH protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Dopamine beta hydroxylase antibody (GTX101616) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Dopamine beta Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX101616

GTX101616 IHC-P Image

Dopamine beta hydroxylase antibody detects DBH protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
Dopamine beta hydroxylase antibody (GTX101616) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169