APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.22 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Dopamine beta Hydroxylase. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950093
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dopamine beta-hydroxylase , DBM , ORTHYP1
Cellular Localization
Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle , secretory vesicle lumen , Cytoplasmic vesicle , secretory vesicle , chromaffin granule lumen , secretory vesicle membrane , chromaffin granule membrane
Background
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101616 IHC-P Image
Dopamine beta hydroxylase antibody detects DBH protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Dopamine beta hydroxylase antibody (GTX101616) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101616 IHC-P Image
Dopamine beta hydroxylase antibody detects DBH protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Dopamine beta hydroxylase antibody (GTX101616) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101616 IHC-P Image
Dopamine beta hydroxylase antibody detects DBH protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
Dopamine beta hydroxylase antibody (GTX101616) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101616 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody (GTX101616) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX101616 IHC-P Image
Dopamine beta Hydroxylase antibody detects Dopamine beta Hydroxylase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody (GTX101616) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101616 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dopamine beta Hydroxylase antibody (GTX101616) diluted at 1:2000 and competitor's antibody diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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REFERENCE
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REVIEW
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