GeneTex

  • Country / Location Selection

United States (US)

Doublecortin antibody [2G5]

Anti-Doublecortin antibody [2G5] used in IHC (Paraffin sections) (IHC-P). GTX60612
Anti-Doublecortin antibody [2G5] used in ELISA (ELISA). GTX60612
Anti-Doublecortin antibody [2G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60612
Anti-Doublecortin antibody [2G5] used in IHC (Paraffin sections) (IHC-P). GTX60612
Anti-Doublecortin antibody [2G5] used in Western Blot (WB). GTX60612
Anti-Doublecortin antibody [2G5] used in Western Blot (WB). GTX60612
Anti-Doublecortin antibody [2G5] used in Flow cytometry (FACS). GTX60612

Cat. No. GTX60612

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2G5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Doublecortin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Doublecortin (AA: 362-411) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887988

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

doublecortin , DBCN , DC , LISX , SCLH , XLIS

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES

Anti-Doublecortin antibody [2G5] used in IHC (Paraffin sections) (IHC-P). GTX60612

GTX60612 IHC-P Image

IHC-P analysis of kidney cancer tissue using GTX60612 Doublecortin antibody [2G5].

Anti-Doublecortin antibody [2G5] used in ELISA (ELISA). GTX60612

GTX60612 ELISA Image

ELISA analysis of antigen using GTX60612 Doublecortin antibody [2G5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Doublecortin antibody [2G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60612

GTX60612 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60612 Doublecortin antibody [2G5].
Green : Doublecortin
Blue: DRAQ5 fluorescent DNA dye

Anti-Doublecortin antibody [2G5] used in IHC (Paraffin sections) (IHC-P). GTX60612

GTX60612 IHC-P Image

IHC-P analysis of human brain tissue using GTX60612 Doublecortin antibody [2G5].

Anti-Doublecortin antibody [2G5] used in Western Blot (WB). GTX60612

GTX60612 WB Image

WB analysis of human DCX recombinant protein using GTX60612 Doublecortin antibody [2G5].

Anti-Doublecortin antibody [2G5] used in Western Blot (WB). GTX60612

GTX60612 WB Image

WB analysis of Mouse heart lysate using GTX60612 Doublecortin antibody [2G5].

Anti-Doublecortin antibody [2G5] used in Flow cytometry (FACS). GTX60612

GTX60612 FACS Image

FACS analysis of SK-N-SH cells using GTX60612 Doublecortin antibody [2G5].
Green : Doublecortin
Red : negative control

REFERENCE

Application Reference

Andr獺s IE et al. Mol Brain 2020; 13 (1) : 21 Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Doublecortin antibody [2G5] (GTX60612). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399