Doublecortin antibody detects Doublecortin protein by western blot analysis. Non-transfected (-) and Doublecortin-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin antibody (GTX113671) at a dilution of 1:10000.