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Doublecortin antibody [N3C3]

Anti-Doublecortin antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107133
Anti-Doublecortin antibody [N3C3] used in Western Blot (WB). GTX107133

Cat. No. GTX107133

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Doublecortin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

human DCX-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Doublecortin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950100

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

doublecortin , DBCN , DC , LISX , SCLH , XLIS

Cellular Localization

Cytoplasm

Background

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Doublecortin antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107133

GTX107133 ICC/IF Image

Immunofluorescent staining of neurons derived from human ESC with a 1:200 dilution of GeneTex anti-doublecortin antibody (GTX107133). Anti-doublecortin antibody (red) colocalizes with anti-MAP2 antibody (green). Image courtesy of Jente Lu, UC Irvine.

Anti-Doublecortin antibody [N3C3] used in Western Blot (WB). GTX107133

GTX107133 WB Image

Doublecortin antibody detects Doublecortin protein by western blot analysis. Non-transfected (-) and Doublecortin-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin antibody (GTX107133) at a dilution of 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169