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Doublecortin antibody

Anti-Doublecortin antibody used in Western Blot (WB). GTX134052
Anti-Doublecortin antibody used in Western Blot (WB). GTX134052
Anti-Doublecortin antibody used in IHC (Frozen sections) (IHC-Fr). GTX134052
Anti-Doublecortin antibody used in Western Blot (WB). GTX134052
Anti-Doublecortin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134052
Anti-Doublecortin antibody used in IHC (Paraffin sections) (IHC-P). GTX134052
Anti-Doublecortin antibody used in Western Blot (WB). GTX134052

Cat No. GTX134052

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

mouse brain , mouse fetal brain , rat brain

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Doublecortin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887191

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

doublecortin , Dbct

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase. Studies in knockout mice lacking this gene and the LIS1 gene suggest that the molecular interaction of these two genes is important in both in neuronal migration and neurogenesis, and there is a cortical role of this gene in nuclear translocation and positioning of the mitotic spindle in radial glial mitotic division. Multiple transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Doublecortin antibody used in Western Blot (WB). GTX134052

GTX134052 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Doublecortin antibody (GTX134052) diluted at 1:1000 and competitor's antibody (MO22113) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-Doublecortin antibody used in Western Blot (WB). GTX134052

GTX134052 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin antibody (GTX134052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Doublecortin antibody used in IHC (Frozen sections) (IHC-Fr). GTX134052

GTX134052 IHC-Fr Image

Doublecortin antibody detects Doublecortin protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: Doublecortin stained by Doublecortin antibody (GTX134052) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Doublecortin antibody used in Western Blot (WB). GTX134052

GTX134052 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin antibody (GTX134052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Doublecortin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134052

GTX134052 ICC/IF Image

Doublecortin antibody detects Doublecortin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Doublecortin stained by Doublecortin antibody (GTX134052) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Doublecortin antibody used in IHC (Paraffin sections) (IHC-P). GTX134052

GTX134052 IHC-P Image

Doublecortin antibody detects Doublecortin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 mouse embryo.
Doublecortin stained by Doublecortin antibody (GTX134052) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Doublecortin antibody used in Western Blot (WB). GTX134052

GTX134052 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin antibody (GTX134052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

Doublecortin antibody Cat No. GTX134052

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Cell Neurosci in 2018. PMID: 30483062
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169