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Doublecortin (phospho Ser335) antibody

Orthogonal
Anti-Doublecortin (phospho Ser335) antibody used in Western Blot (WB). GTX03587
Anti-Doublecortin (phospho Ser335) antibody used in Western Blot (WB). GTX03587
Anti-Doublecortin (phospho Ser335) antibody used in ELISA (ELISA). GTX03587
Anti-Doublecortin (phospho Ser335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03587

Cat. No. GTX03587

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($409)
See all Doublecortin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
IHC-P 1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Doublecortin around the phosphorylation site of Ser335.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

doublecortin , DBCN , DC , LISX , SCLH , XLIS

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES

Anti-Doublecortin (phospho Ser335) antibody used in Western Blot (WB). GTX03587

GTX03587 WB Image

WB analysis of mouse lung tissue lysate using GTX03587 Doublecortin (phospho Ser335) antibody. The lane on the left was treated with the antigen-specific peptide.

Anti-Doublecortin (phospho Ser335) antibody used in Western Blot (WB). GTX03587

GTX03587 WB Image

WB analysis of K562 cell lysate using GTX03587 Doublecortin (phospho Ser335) antibody.

Anti-Doublecortin (phospho Ser335) antibody used in ELISA (ELISA). GTX03587

GTX03587 ELISA Image

Orthogonal

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX03587 Doublecortin (phospho Ser335) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-Doublecortin (phospho Ser335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03587

GTX03587 IHC-P Image

IHC-P analysis of rat brain tissue using GTX03587 Doublecortin (phospho Ser335) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 409