GeneTex
United States (US)

Drebrin antibody [M2F6]

Drebrin-antibody-M2F6_WB_GTX12350-1_18121410_592.jpg

Cat No. GTX12350

Host Mouse
Clonality Monoclonal
Clone Name M2F6
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Cat, Guinea pig, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

Mouse Brain Tissue Extract

Specificity/Sensitivity

This antibody detects Drebrin A and E in samples from human, mouse, rat, guinea pig, and cat origins and Drebrin E only in chicken samples.
PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: PBS, 10mg/ml Sucrose. pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Chicken

Immunogen

Chicken Drebrin E protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Cytoplasmic

Background

The cortical cytoskeleton, a highly cross-linked submembranal F-actin filament network, plays an important role in cell proliferation, differentiation, migration, cell morphology and oncogenic transformation. Drebrin (developmentally-regulated brain protein) is a major neuronal F-actin binding protein involved in the control of actin dynamics and neuronal morphogenesis. Three major isoforms of drebrin, resulting from alternative splicing of a single gene, have been identified in the brain: the embryonic isoforms designated E1 and E2 and the adult or A isoform (95-120 kDa). An additional truncated form of drebrin A, s-drebrin/A2 (42 kDa), is specifically expressed in the adult brain, but not in non-neuronal tissues. Drebrin is also widely expressed in a variety of cells including epithelial, endothelial, and smooth muscle cells and is associated at cell-cell adhering junction sites.
Show More +

Database

Research Area

DATA IMAGES
Drebrin-antibody-M2F6_WB_GTX12350-1_18121410_592.jpg

GTX12350 WB Image

Western blot analysis of Drebrin expression in mouse brain (1) NIH/3T3 (2), Jurkat (3), HeLa (4), and PC12 (5) using Drebrin mAb (M2F6).

Reference