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Drebrin antibody

Anti-Drebrin antibody used in Western Blot (WB). GTX111649
Anti-Drebrin antibody used in Western Blot (WB). GTX111649
Anti-Drebrin antibody used in IHC (Paraffin sections) (IHC-P). GTX111649
Anti-Drebrin antibody used in IHC (Paraffin sections) (IHC-P). GTX111649
Anti-Drebrin antibody used in Western Blot (WB). GTX111649

Cat. No. GTX111649

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Drebrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Observed MW

125 kDa. The observed M.W. is based on the following publication. PMID: 23979715

Positive Control

IMR32 , SK-N-SH , IMR-32 , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Drebrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175514

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

drebrin 1 , D0S117E

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Drebrin antibody used in Western Blot (WB). GTX111649

GTX111649 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX111649 diluted at 1:10000

Explanation note：The observed M.W. (~125kDa) is based on the publication: PMID: 23979715

Anti-Drebrin antibody used in Western Blot (WB). GTX111649

GTX111649 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Drebrin antibody (GTX111649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Drebrin antibody used in IHC (Paraffin sections) (IHC-P). GTX111649

GTX111649 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Drebrin(GTX111649) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Drebrin antibody used in IHC (Paraffin sections) (IHC-P). GTX111649

GTX111649 IHC-P Image

Drebrin antibody detects Drebrin protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Drebrin antibody (GTX111649) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Drebrin antibody used in Western Blot (WB). GTX111649

GTX111649 WB Image

Various whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Drebrin antibody (GTX111649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169