GeneTex

  • Country / Location Selection

United States (US)

Drosha antibody [GT1178]

Recombinant antibody Orthogonal
Anti-Drosha antibody [GT1178] used in Western Blot (WB). GTX09511
Anti-Drosha antibody [GT1178] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX09511
Anti-Drosha antibody [GT1178] used in Western Blot (WB). GTX09511

Cat. No. GTX09511

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1178

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all Drosha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

159 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan Drosha

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

drosha ribonuclease III , ETOHI2 , HSA242976 , RANSE3L , RN3 , RNASE3L , RNASEN

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a hmuan cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in hmuan patients with kidney cancer. [provided by RefSeq, Sep 2016]

Database

DATA IMAGES

Anti-Drosha antibody [GT1178] used in Western Blot (WB). GTX09511

GTX09511 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Drosha antibody [GT1178] (GTX09511) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed M.W. is based on the publication: PMID: 27471035

Anti-Drosha antibody [GT1178] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX09511

GTX09511 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX09511 Drosha antibody [GT1178].
Orange: Primary antibody
Blue: DAPI

Anti-Drosha antibody [GT1178] used in Western Blot (WB). GTX09511

GTX09511 WB Image

WB analysis of various samples using GTX09511 Drosha antibody [GT1178].
Dilution : 1:1000
Loading : 25 μg

REFERENCE

There are currently no references for Drosha antibody [GT1178] (GTX09511). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Drosha antibody [GT1178] (GTX09511). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399