APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89198-PEP).
Product Note
This antibody also cross-reacts with human Ribonuclease III (GeneID 29102; NP_037367.2).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Peptide with sequence C-KQTDKQKLAQRE, from the internal region of the protein sequence according to NP_081075.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
drosha ribonuclease III , ETOHI2 , HSA242976 , RANSE3L , RN3 , RNASE3L , RNASEN
Cellular Localization
Nucleus , Nucleolus
Background
This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]
Database
DATA IMAGES
|
GTX89198 WB Image
WB analysis of human liver lysate using GTX89198 Drosha antibody, Internal. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for Drosha antibody, Internal (GTX89198). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Drosha antibody, Internal (GTX89198). Be the first to share your experience with this product.