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DsbA antibody [1A2-D4-G10]

Anti-DsbA antibody [1A2-D4-G10] used in Western Blot (WB). GTX49173
Anti-DsbA antibody [1A2-D4-G10] used in Western Blot (WB). GTX49173

Cat No. GTX49173

Host

Mouse

Clonality

Monoclonal

Clone Name

1A2-D4-G10

Isotype

IgG2b

Application

WB

Reactivity

E. coli
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human DsbA protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

iarA Antibody , dsbA Antibody , ppfA Antibody , JW3832 Antibody , ECK3852 Antibody , dsf Antibody

Cellular Localization

Periplasm

Background

Disulphide oxidoreductase (DsbA) is the major oxidase responsible for generation of disulfide bonds in proteins of E. coli envelope. It is a member of the thioredoxin superfamily. DsbA introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA is reoxidized by dsbB. It is required for pilus biogenesis.

Research Area

DATA IMAGES

Anti-DsbA antibody [1A2-D4-G10] used in Western Blot (WB). GTX49173

GTX49173 WB Image

WB analysis of DsbA recombinant protein using DsbA antibody.

Anti-DsbA antibody [1A2-D4-G10] used in Western Blot (WB). GTX49173

GTX49173 WB Image

Western blot detection of DSBA in AKT1,AKT2,AKT3 and DSBA recombinant antigen fragments using DSBA antibody (1:1000 diluted).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf