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Dvl2 antibody

Anti-Dvl2 antibody used in Immunohistochemistry (IHC). GTX129968

Cat No. GTX129968

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC 1:100-1:1000
Not tested in other applications.

Positive Control

zebrafish retina

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Dvl2

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dishevelled segment polarity protein 2 , sb:eu471 , wu:fc05d12 , wu:fo71e09 , wu:fp54a02 , zgc:55372

Database

Research Area

DATA IMAGES

Anti-Dvl2 antibody used in Immunohistochemistry (IHC). GTX129968

GTX129968 IHC Image

cytoplasm in the photoreceptor
Immunohistochemistry of flat-mounted zebrafish retina using an antibody against dvl2 (GTX129968) at a 1:200 dilution.

REFERENCE

REVIEW

Dvl2 antibody Cat No. GTX129968

Rating ( Average 4 based on 1 users reviews)
Application
Immunohistochemistry(IHC) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 10-Jul-2013
Score :
Application Tested : IHC
Sample Species : Zfsh
Sample : flat-mounted zebrafish retina
Blocking : 10% NGS/ 1% triton X-100/ 1% Tween 20/ 1% DMSO in PBS with 0.1% sodium azide
Fixation : 4% paraformaldehyde in 0.1M phosphate buffer (Ph7.4) with 5% sucrose
Permeabilization /Antigen retrieval : 10 mM sodium citrate/ 0.05% tween (pH6.0), 98°C, 10 minHr
Primary Antibody : 1:200, 25°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 409
$ 219