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Dvl2 antibody

Anti-Dvl2 antibody used in Immunohistochemistry (IHC). GTX129968

Cat No. GTX129968

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC 1:100-1:1000
Not tested in other applications.

Positive Control

zebrafish retina

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Dvl2

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886141

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dishevelled segment polarity protein 2 , sb:eu471 , wu:fc05d12 , wu:fo71e09 , wu:fp54a02 , zgc:55372

Database

Research Area

DATA IMAGES

Anti-Dvl2 antibody used in Immunohistochemistry (IHC). GTX129968

GTX129968 IHC Image

cytoplasm in the photoreceptor
Immunohistochemistry of flat-mounted zebrafish retina using an antibody against dvl2 (GTX129968) at a 1:200 dilution.

REFERENCE

REVIEW

Dvl2 antibody Cat No. GTX129968

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Zfsh
Figure Note : The data was pear reviewed and published in the journal J Cell Sci in 2014.PMID: 25074807
Date : Anonymous submitted on 10-Jul-2013
Score :
Application Tested : IHC
Sample Species : Zfsh
Sample : flat-mounted zebrafish retina
Blocking : 10% NGS/ 1% triton X-100/ 1% Tween 20/ 1% DMSO in PBS with 0.1% sodium azide
Fixation : 4% paraformaldehyde in 0.1M phosphate buffer (Ph7.4) with 5% sucrose
Permeabilization /Antigen retrieval : 10 mM sodium citrate/ 0.05% tween (pH6.0), 98°C, 10 minHr
Primary Antibody : 1:200, 25°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 409
$ 219