GeneTex
United States (US)

Dynamin 1 antibody [4E67]

Cat No. GTX14448

Host Mouse
Clonality Monoclonal
Clone Name 4E67
Isotype IgG
Application WB, IP, IHC
Reactivity Human, Mouse, Rat, Bovine
APPLICATION

Application Note

IHC: Use at an assay dependent dilution. Staining of formaldehyde perfused and fixed rat brain shows synaptic immunofluorescence. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 mg/ml. Predicted molecular weight: 97 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

97 kDa. ( Note )

Positive Control

Mouse Brain Tissue Extract
PROPERTIES

Form

Liquid

Buffer

Preservative: None; Constituents: 0.1mM PMSF, PBS, 40% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment, (His)6 tagged, corresponding to amino acids 1-750 of Human Dynamin 1.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dynamin 1,Dnm,Eiee31,Dnm1

Cellular Localization

Microtubule associated.

Background

Dynamin is a 100 kDa GTP binding protein that was first identified as a protein able to bind microtubules. A mutation in the Drosophila gene shits, encoding a dynamin homologue, prevents synaptic vesicle recycling and causes temperature-sensitive paralysis, implicating dynamin in the formation of coated vesicles. Dynamin may mediate vesicle formation by assembling into a ring structure around the necks of invaginated coated pits that may eventually lead to the closure of the neck and formation of vesicles. Amphiphysin, dynamin, clathrin and AP2 form a complex during synaptic vesicle endocytosis, which is regulated by the phosphorylated state of different components in this complex. To date, three genes have been found to encode dynamin isoforms. Dynamin 1 is neuronal specific, Dynamin 2 is ubiquitously expressed and Dynamin 3 localizes to testis, brain and lung.

Database

Research Area

Package List Price ($)
$ 319