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Dynamin 1 antibody [4E67]

Anti-Dynamin 1 antibody [4E67] used in Western Blot (WB). GTX14448

Cat. No. GTX14448

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E67

Isotype

 IgG1

Application

 WB, FACS, IP, IHC

Reactivity

 Human, Mouse, Rat, Bovine, Chicken
Package
50 μg ($399)
See all Dynamin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5μg/ml
FACS 10μg/ml
IP Assay dependent
IHC 1:50
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 0.1mM PMSF, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant His 6-tagged human dynamin (aa1-750). Epitope: GNRGMEDLIPLVNRLQD.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNM , DNM1 , EIEE31 , dynamin 1 , Dynamin 1

Cellular Localization

Cytoplasm,Cytoskeleton

Background

This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Dynamin 1 antibody [4E67] used in Western Blot (WB). GTX14448

GTX14448 WB Image

WB analysis of SL29 cell lysate using GTX14448 Dynamin 1 antibody [4E67].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399