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Dynamin 2 antibody [C2C3], C-term

KO/KD
Anti-Dynamin 2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109652
Anti-Dynamin 2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109652
Anti-Dynamin 2 antibody [C2C3], C-term used in Western Blot (WB). GTX109652
Anti-Dynamin 2 antibody [C2C3], C-term used in Western Blot (WB). GTX109652
Anti-Dynamin 2 antibody [C2C3], C-term used in Western Blot (WB). GTX109652
Anti-Dynamin 2 antibody [C2C3], C-term used in Western Blot (WB). GTX109652

Cat. No. GTX109652

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Dynamin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Dynamin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950134

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dynamin 2 , CMT2M , CMTDI1 , CMTDIB , DI-CMTB , DYN2 , DYNII , LCCS5

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density

Background

Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Four alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Dynamin 2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109652

GTX109652 ICC/IF Image

Dynamin 2 antibody [C2C3], C-term detects DNM2 protein at cytoplasm by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DNM2 protein stained by Dynamin 2 antibody [C2C3], C-term (GTX109652) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Anti-Dynamin 2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109652

GTX109652 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Dynamin 2(GTX109652) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Dynamin 2 antibody [C2C3], C-term used in Western Blot (WB). GTX109652

GTX109652 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
7.5% SDS PAGE
GTX109652 diluted at 1:1000

Anti-Dynamin 2 antibody [C2C3], C-term used in Western Blot (WB). GTX109652

GTX109652 WB Image

KO/KD

The data was published in the Elife in 2019. PMID: 30648534

Anti-Dynamin 2 antibody [C2C3], C-term used in Western Blot (WB). GTX109652

GTX109652 WB Image

KO/KD

The data was published in the Elife in 2019. PMID: 30648534

Anti-Dynamin 2 antibody [C2C3], C-term used in Western Blot (WB). GTX109652

GTX109652 WB Image

KO/KD

The data was published in the Elife in 2019. PMID: 30648534

REFERENCE

Application Reference

Gerber D et al. Elife 2019; 8 Schwann cells, but not Oligodendrocytes, Depend Strictly on Dynamin 2 Function.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169