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Dynamin 2 antibody [N1N3]

KO/KD
Anti-Dynamin 2 antibody [N1N3] used in Western Blot (WB). GTX113171
Anti-Dynamin 2 antibody [N1N3] used in Western Blot (WB). GTX113171
Anti-Dynamin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113171
Anti-Dynamin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113171
Anti-Dynamin 2 antibody [N1N3] used in Western Blot (WB). GTX113171
Anti-Dynamin 2 antibody [N1N3] used in Western Blot (WB). GTX113171
Anti-Dynamin 2 antibody [N1N3] used in Western Blot (WB). GTX113171

Cat. No. GTX113171

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Dynamin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

mouse brain , PC-12 , Rat-2 , rat brain

Predict Reactivity

Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dynamin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722896

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dynamin 2 , CMT2M , CMTDI1 , CMTDIB , DI-CMTB , DYN2 , DYNII , LCCS5

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density

Background

Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Four alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Dynamin 2 antibody [N1N3] used in Western Blot (WB). GTX113171

GTX113171 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody (GTX113171) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dynamin 2 antibody [N1N3] used in Western Blot (WB). GTX113171

GTX113171 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody [N1N3] (GTX113171) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dynamin 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113171

GTX113171 IHC-P Image

Dynamin 2 antibody [N1N3] detects Dynamin 2 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Dynamin 2 antibody [N1N3] (GTX113171) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Dynamin 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113171

GTX113171 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Dynamin 2(GTX113171) antibody at 1:500 dilution.

Anti-Dynamin 2 antibody [N1N3] used in Western Blot (WB). GTX113171

GTX113171 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody [N1N3] (GTX113171) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dynamin 2 antibody [N1N3] used in Western Blot (WB). GTX113171

GTX113171 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody [N1N3] (GTX113171) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dynamin 2 antibody [N1N3] used in Western Blot (WB). GTX113171

GTX113171 WB Image

KO/KD

The data was published in the journal Elife in 2019.PMID: 30801249

REFERENCE

Application Reference

Rivera-Serrano EE et al. Elife 2019; 8 Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses.
Loh LN et al. Traffic 2017; (Epub) Escherichia coli K1 utilises host macropinocytic pathways for invasion of brain microvascular endothelial cells.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169