GeneTex
United States (US)

Dynamin antibody [0.T.178]

Cat No. GTX14447

Host Mouse
Clonality Monoclonal
Clone Name 0.T.178
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat, Bovine
APPLICATION

Application Note

ICC: Use at a concentration of 10 μg/ml. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.1 - 0.2 μg/ml. Predicted molecular weight: 100 kDa. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

Bovine brain cytosol
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.05% Sodium Azide; Constituents: PBS, 40 % glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Microtubule associated.

Background

Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.

Research Area

Package List Price ($)
$ 289