APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , H1299 , HepG2 , Raji , mouse liver
Predict Reactivity
Rat, Bovine, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.68mg/ml
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Dyrk3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
DYRK5 antibody, RED antibody, REDK antibody, hYAK3-2 antibody, DYRK3 antibody, regulatory erythroid kinase antibody, protein kinase Dyrk3 antibody, dual specificity tyrosine-phosphorylation-regulated kinase 3 antibody, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 5 antibody, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 antibody
Cellular Localization
Nucleus
Background
This gene product belongs to the DYRK family of dual-specificity protein kinases that catalyze autophosphorylation on serine/threonine and tyrosine residues. The members of this family share structural similarity, however, differ in their substrate specificity, suggesting their involvement in different cellular functions. The encoded protein has been shown to autophosphorylate on tyrosine residue and catalyze phosphorylation of histones H3 and H2B in vitro. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
Database
DATA IMAGES
|
GTX107889 WB Image
Sample (30μg whole cell lysate)
A:Raji (GTX27908)
7.5% SDS PAGE
GTX107889 diluted at 1:1000
|
|
GTX107889 WB Image
Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX107889 diluted at 1:1000
|
