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Dystrophin antibody [Dy10/12B2]

Cat. No. GTX01869

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Dy10/12B2

Isotype

 IgG2a

Application

 IHC-Fr

Reactivity

 Human
Package
500 μl ($399)
See all Dystrophin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Neat-1:20

Note :

IHC-Fr
Freeze specimen tissue blocks in isopentane chilled in liquid nitrogen.
Not tested in other applications.

Calculated MW

427 kDa. ( Note )

Product Note

This antibody reacts strongly with the amino terminal domain (between amino acids 321 and 494) of human dystrophin. Patient immunoreactivity indicates epitope is near exons 10 to 12. Epitope mapping suggests that sequences from amino acids 308 to 351 are involved in antibody binding. This region spans the junction of exons 9 and 10 and the epitope recognised may be part of a hinge region joining the amino domain to the central rod domain. No reactivity with DMD/BMD patients deleted for exons 10 to 12.
We do not recommend use of this product for Mouse,Rat,Rabbit,Dog,Hamster,Chicken,Pig samples.

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Fusion protein containing amino acids 67 to 713.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dystrophin , BMD , CMD3B , DXS142 , DXS164 , DXS206 , DXS230 , DXS239 , DXS268 , DXS269 , DXS270 , DXS272 , MRX85

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene spans a genomic range of greater than 2 Mb and encodes a large protein containing an N-terminal actin-binding domain and multiple spectrin repeats. The encoded protein forms a component of the dystrophin-glycoprotein complex (DGC), which bridges the inner cytoskeleton and the extracellular matrix. Deletions, duplications, and point mutations at this gene locus may cause Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), or cardiomyopathy. Alternative promoter usage and alternative splicing result in numerous distinct transcript variants and protein isoforms for this gene. [provided by RefSeq, Dec 2016]

Database

Research Area

REFERENCE

Application Reference

Hindi SM et al. bioRxiv 2023; Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery.
Application : IHC-Fr
Reactivity : Mouse
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REVIEW

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Package List Price ($)
$ 399